| Game Results |
| Teams | Date | Score |
| Boston at Buffalo | 9/11/1965 | BUA 23-6 |
| Denver at San Diego | 9/11/1965 | DEA 24-16 |
| Kansas City at Oakland | 9/12/1965 | KCA 25-10 |
| New York AFL at Houston | 9/12/1965 | HOA 26-21 |
| Kansas City at New York AFL | 9/18/1965 | KCA 31-24 |
| Chicago at San Francisco | 9/19/1965 | SFN 23-21 |
| Los Angeles at Detroit | 9/19/1965 | LAN 24-20 |
| Minnesota at Baltimore | 9/19/1965 | BAN 26-17 |
| New York NFL at Dallas | 9/19/1965 | NYN 35-19 |
| Green Bay at Pittsburgh | 9/19/1965 | GBN 27-3 |
| St. Louis at Philadelphia | 9/19/1965 | SLN 17-17 |
| Cleveland at Washington | 9/19/1965 | WAN 38-27 |
| San Diego at Oakland | 9/19/1965 | SDA 15-7 |
| Buffalo at Denver | 9/19/1965 | BUA 27-20 |
| Boston at Houston | 9/19/1965 | HOA 33-24 |
| Denver at Boston | 9/24/1965 | DEA 34-25 |
| Chicago at Los Angeles | 9/25/1965 | LAN 28-7 |
| New York AFL at Buffalo | 9/26/1965 | BUA 28-10 |
| Houston at Oakland | 9/26/1965 | HOA 28-25 |
| Kansas City at San Diego | 9/26/1965 | SDA 31-0 |
| Washington at Dallas | 9/26/1965 | WAN 24-0 |
| Pittsburgh at San Francisco | 9/26/1965 | SFN 26-16 |
| New York NFL at Philadelphia | 9/26/1965 | NYN 33-21 |
| Detroit at Minnesota | 9/26/1965 | MNN 24-21 |
| St. Louis at Cleveland | 9/26/1965 | SLN 13-7 |
| Baltimore at Green Bay | 9/26/1965 | BAN 28-14 |
| Chicago at Green Bay | 10/3/1965 | GBN 34-28 |
| Minnesota at Los Angeles | 10/3/1965 | LAN 27-14 |
| Cleveland at Philadelphia | 10/3/1965 | CLN 35-31 |
| New York NFL at Pittsburgh | 10/3/1965 | NYN 35-16 |
| San Francisco at Baltimore | 10/3/1965 | SFN 10-9 |
| Washington at Detroit | 10/3/1965 | DEN 14-10 |
| Houston at San Diego | 10/3/1965 | SDA 31-9 |
| New York AFL at Denver | 10/3/1965 | NYA 19-16 |
| Boston at Kansas City | 10/3/1965 | BOA 19-13 |
| Oakland at Buffalo | 10/3/1965 | OAA 44-20 |
| Dallas at St. Louis | 10/4/1965 | SLN 37-3 |
| Oakland at Boston | 10/8/1965 | OAA 28-14 |
| Pittsburgh at Cleveland | 10/9/1965 | CLN 30-17 |
| San Diego at Buffalo | 10/10/1965 | SDA 52-7 |
| Kansas City at Denver | 10/10/1965 | KCA 22-6 |
| St. Louis at Washington | 10/10/1965 | WAN 6-3 |
| San Francisco at Green Bay | 10/10/1965 | GBN 20-7 |
| New York NFL at Minnesota | 10/10/1965 | MNN 31-14 |
| Los Angeles at Chicago | 10/10/1965 | CHN 27-21 |
| Detroit at Baltimore | 10/10/1965 | BAN 35-34 |
| Philadelphia at Dallas | 10/10/1965 | PHN 40-20 |
| Oakland at New York AFL | 10/16/1965 | NYA 34-6 |
| Dallas at Cleveland | 10/17/1965 | CLN 30-28 |
| Green Bay at Detroit | 10/17/1965 | GBN 20-10 |
| San Francisco at Los Angeles | 10/17/1965 | SFN 31-17 |
| Chicago at Minnesota | 10/17/1965 | CHN 38-10 |
| Philadelphia at New York NFL | 10/17/1965 | NYN 28-16 |
| St. Louis at Pittsburgh | 10/17/1965 | SLN 33-26 |
| Baltimore at Washington | 10/17/1965 | BAN 45-14 |
| Houston at Denver | 10/17/1965 | HOA 26-22 |
| Buffalo at Kansas City | 10/17/1965 | BUA 10-10 |
| San Diego at Boston | 10/17/1965 | SDA 30-3 |
| San Diego at New York AFL | 10/23/1965 | SDA 35-27 |
| Denver at Buffalo | 10/24/1965 | DEA 20-17 |
| Kansas City at Houston | 10/24/1965 | KCA 27-13 |
| Boston at Oakland | 10/24/1965 | OAA 20-16 |
| Minnesota at San Francisco | 10/24/1965 | MNN 41-37 |
| Washington at St. Louis | 10/24/1965 | WAN 14-13 |
| Pittsburgh at Philadelphia | 10/24/1965 | PHN 37-10 |
| Cleveland at New York NFL | 10/24/1965 | CLN 24-21 |
| Los Angeles at Baltimore | 10/24/1965 | BAN 27-23 |
| Detroit at Chicago | 10/24/1965 | DEN 24-21 |
| Dallas at Green Bay | 10/24/1965 | GBN 21-16 |
| Minnesota at Cleveland | 10/31/1965 | MNN 36-34 |
| Dallas at Pittsburgh | 10/31/1965 | DAN 28-15 |
| Green Bay at Chicago | 10/31/1965 | CHN 10-6 |
| Detroit at Los Angeles | 10/31/1965 | LAN 31-7 |
| Philadelphia at Washington | 10/31/1965 | WAN 26-7 |
| St. Louis at New York NFL | 10/31/1965 | SLN 23-21 |
| Baltimore at San Francisco | 10/31/1965 | SFN 28-23 |
| Boston at San Diego | 10/31/1965 | SDA 38-17 |
| Oakland at Kansas City | 10/31/1965 | OAA 17-17 |
| Houston at Buffalo | 10/31/1965 | HOA 17-17 |
| Denver at New York AFL | 10/31/1965 | NYA 24-7 |
| Buffalo at Boston | 11/7/1965 | BUA 20-13 |
| Oakland at Houston | 11/7/1965 | OAA 35-10 |
| New York AFL at Kansas City | 11/7/1965 | NYA 16-14 |
| San Diego at Denver | 11/7/1965 | SDA 23-16 |
| San Francisco at Dallas | 11/7/1965 | SFN 24-20 |
| Pittsburgh at St. Louis | 11/7/1965 | SLN 24-12 |
| Philadelphia at Cleveland | 11/7/1965 | PHN 21-16 |
| Washington at New York NFL | 11/7/1965 | NYN 28-20 |
| Los Angeles at Minnesota | 11/7/1965 | MNN 38-14 |
| Detroit at Green Bay | 11/7/1965 | DEN 17-10 |
| Baltimore at Chicago | 11/7/1965 | BAN 20-10 |
| San Francisco at Detroit | 11/14/1965 | SFN 17-6 |
| Los Angeles at Green Bay | 11/14/1965 | LAN 21-16 |
| Baltimore at Minnesota | 11/14/1965 | BAN 20-0 |
| New York NFL at Cleveland | 11/14/1965 | NYN 21-20 |
| Pittsburgh at Dallas | 11/14/1965 | DAN 13-3 |
| St. Louis at Chicago | 11/14/1965 | CHN 40-10 |
| Washington at Philadelphia | 11/14/1965 | WAN 24-3 |
| Buffalo at Oakland | 11/14/1965 | OAA 21-7 |
| San Diego at Kansas City | 11/14/1965 | SDA 16-14 |
| Denver at Houston | 11/14/1965 | DEA 36-17 |
| New York AFL at Boston | 11/14/1965 | BOA 13-3 |
| Kansas City at Boston | 11/21/1965 | KCA 20-20 |
| Houston at New York AFL | 11/21/1965 | HOA 30-28 |
| Oakland at Denver | 11/21/1965 | DEA 22-21 |
| New York NFL at St. Louis | 11/21/1965 | SLN 38-30 |
| Washington at Pittsburgh | 11/21/1965 | WAN 54-0 |
| Philadelphia at Baltimore | 11/21/1965 | BAN 31-14 |
| Green Bay at Minnesota | 11/21/1965 | MNN 20-14 |
| Los Angeles at San Francisco | 11/21/1965 | LAN 36-31 |
| Chicago at Detroit | 11/21/1965 | CHN 42-24 |
| Cleveland at Dallas | 11/21/1965 | DAN 33-27 |
| Baltimore at Detroit | 11/25/1965 | DEN 24-20 |
| Buffalo at San Diego | 11/25/1965 | SDA 30-10 |
| Dallas at Washington | 11/28/1965 | WAN 31-28 |
| Green Bay at Los Angeles | 11/28/1965 | GBN 24-23 |
| San Francisco at Minnesota | 11/28/1965 | SFN 45-24 |
| Chicago at New York NFL | 11/28/1965 | CHN 28-21 |
| Cleveland at Pittsburgh | 11/28/1965 | CLN 24-6 |
| Philadelphia at St. Louis | 11/28/1965 | PHN 42-38 |
| Houston at Kansas City | 11/28/1965 | KCA 34-17 |
| Boston at New York AFL | 11/28/1965 | NYA 28-20 |
| New York AFL at San Diego | 12/4/1965 | SDA 22-15 |
| Buffalo at Houston | 12/5/1965 | HOA 28-10 |
| Denver at Oakland | 12/5/1965 | OAA 34-22 |
| Washington at Cleveland | 12/5/1965 | WAN 38-28 |
| Los Angeles at St. Louis | 12/5/1965 | SLN 30-20 |
| Pittsburgh at New York NFL | 12/5/1965 | NYN 45-6 |
| Dallas at Philadelphia | 12/5/1965 | DAN 24-21 |
| Minnesota at Green Bay | 12/5/1965 | GBN 27-21 |
| Detroit at San Francisco | 12/5/1965 | DEN 24-14 |
| Chicago at Baltimore | 12/5/1965 | CHN 28-14 |
| St. Louis at Dallas | 12/11/1965 | SLN 31-20 |
| Kansas City at Buffalo | 12/12/1965 | KCA 19-14 |
| San Diego at Houston | 12/12/1965 | SDA 31-14 |
| Boston at Denver | 12/12/1965 | DEA 35-15 |
| New York AFL at Oakland | 12/12/1965 | OAA 27-19 |
| New York NFL at Washington | 12/12/1965 | WAN 40-28 |
| San Francisco at Chicago | 12/12/1965 | CHN 35-24 |
| Philadelphia at Pittsburgh | 12/12/1965 | PHN 34-6 |
| Minnesota at Detroit | 12/12/1965 | MNN 26-24 |
| Cleveland at Los Angeles | 12/12/1965 | LAN 19-13 |
| Green Bay at Baltimore | 12/12/1965 | GBN 26-20 |
| Baltimore at Los Angeles | 12/18/1965 | LAN 27-24 |
| Houston at Boston | 12/18/1965 | BOA 28-14 |
| Minnesota at Chicago | 12/19/1965 | CHN 35-21 |
| Dallas at New York NFL | 12/19/1965 | NYN 28-24 |
| Detroit at Philadelphia | 12/19/1965 | DEN 20-14 |
| Pittsburgh at Washington | 12/19/1965 | PIN 27-21 |
| Cleveland at St. Louis | 12/19/1965 | SLN 26-14 |
| Green Bay at San Francisco | 12/19/1965 | GBN 24-12 |
| Oakland at San Diego | 12/19/1965 | SDA 37-21 |
| Denver at Kansas City | 12/19/1965 | DEA 31-28 |
| Buffalo at New York AFL | 12/19/1965 | BUA 25-24 |